Izvestiya Vysshikh Uchebnykh Zavedenii. Elektronika home

Publication of the journal

The section is currently being updated

The study of individual living cells of an organism and cell colonies in their natural environment is of great importance for understanding cellular processes and phenotypes. Dissimilar to traditional methods, scanning ion-conducting microscopy (SICM) and surface-enhanced Raman spectroscopy (SERS) allow obtaining information about minor changes in the chemical composition and mechanical characteristics of cells. In this work, the characteristics of SERS-active capillaries based on Ag nanoparticles (NPs) are investigated, the mechanical characteristics of cells are studied, and the dynamics of capillary properties degradation under various storage conditions were revealed. Scanning electron microscopy has shown that at 50 nm of curvature of the capillary tip, the nanoparticle array exhibits the highest density and gain of the order of 105. The topography and mechanical properties of SH-SY5Y cells were scanned using a SERS-active capillary by the SICM method. By means of SERS spectroscopy, it has been established that both in air and in a vacuum desiccator, the degradation process of Ag NPs proceeds in 1 month, after which it stops.

Key words: nanocapillaries, scanning ion-conductance microscopy, surface-enhanced Raman spectroscopy, silver nanoparticles
Published in: BIOMEDICAL ELECTRONICS
Bibliography link: Novikov D. V., Chumachenko J. V., Dubkov S. V., Kolmogorov V. S., Gorelkin P. V., Erofeev A. S. et al. Investigation of the degradation process of SERS-active capillaries for complex use in Raman spectroscopy and scanning ion conductance microscopy. Izv. vuzov. Elektronika = Proc. Univ. Electronics. 2025;30(6):741–753. (In Russ.). https://doi.org/10.24151/1561-5405-2025-30-6-741-753.
Financial source: the work has been supported by the Russian Science Foundation (grant no. 22-19-00824) and within the framework of the state assignment (2024-2026 FSMR-2024-0012 Supplementary agreement no. 075-03-2024-061/3).

Denis V. Novikov
National Research University of Electronic Technology, Russia, 124498, Moscow, Zelenograd, Shokin sq., 1

Julia V. Chumachenko
National Research University of Electronic Technology, Russia, 124498, Moscow, Zelenograd, Shokin sq., 1

Sergey V. Dubkov
National Research University of Electronic Technology, Russia, 124498, Moscow, Zelenograd, Shokin sq., 1

Vasiliy S. Kolmogorov
National University of Science and Technology “MISiS”, Russia, 119049, Moscow, Leninsky ave., 4; Lomonosov Moscow State University, Russia, Moscow, 119991, Kolmogorov st., 1

Peter V. Gorelkin
National University of Science and Technology “MISiS”, Russia, 119049, Moscow, Leninsky ave., 4

Alexander S. Erofeev
National University of Science and Technology “MISiS”, Russia, 119049, Moscow, Leninsky ave., 4

Yuri N. Parkhomenko
National University of Science and Technology “MISiS”, Russia, 119049, Moscow, Leninsky ave., 4

Anastasia V. Zheleznyakova
National Research University of Electronic Technology, Russia, 124498, Moscow, Zelenograd, Shokin sq., 1

Vitaliy F. Popenko
National Research University of Electronic Technology, Russia, 124498, Moscow, Zelenograd, Shokin sq., 1

1. Korchev Yu. E., Gorelik J., Lab M. J., Sviderskaya E. V., Johnston C. L., Coombes Ch. R. et al. Cell volume measurement using scanning ion conductance microscopy. Biophys. J. 2000;78(1):451–457. https://doi.org/10.1016/S0006-3495(00)76607-0

2. Gorelkin P., Erofeev A., Kolmogorov V., Efremov Yu., Novak P., Shevchuk A. et al. Scanning ion conductance microscopy (SICM) for low-stress directly examining of cellular mechanics. Microsc. Microanal. 2020;26(S2):1968–1970. https://doi.org/10.1017/S1431927620019972

3. Zhuang X., Bartley L. E., Babcock H. P., Russell R., Ha T., Herschlag D., Chu S. A single-molecule study of RNA catalysis and folding. Science. 2000;288(5473):2048–2051. https://doi.org/10.1126/science.288.5473.2048

4. Xie X. S., Yu J., Yang W. Y. Living cells as test tubes. Science. 2006;312(5771):228–230. https://doi.org/10.1126/science.1127566

5. Takahashi Y., Sasaki Y., Yoshida T., Honda K., Zhou Yu., Miyamoto T. et al. Nanopipette fabrication guidelines for SICM nanoscale imaging. Anal. Chem. 2023;95(34):12664–12672. https://doi.org/10.1021/acs.analchem.3c01010

6. Sarkar S., Mandler D. Scanning electrochemical microscopy versus scanning ion conductance microscopy for surface patterning. ChemElectroChem. 2017;4(11):2981–2988. https://doi.org/10.1002/celc.201700719

7. Schäffer T. E., Anczykowski B., Fuchs H. Scanning ion conductance microscopy. In: Bhushan B., Fuchs H. (eds). Applied scanning probe methods II: Scanning probe microscopy techniques. Berlin; Heidelberg: Springer; 2022, pp. 91–119. https://doi.org/10.1007/3-540-27453-7_3

8. Seifert J., Rheinlaender J., Novak P., Korchev Yu. E., Schäffer T. E. Comparison of atomic force microscopy and scanning ion conductance microscopy for live cell imaging. Langmuir. 2015;31(24):6807–6813. https://doi.org/10.1021/acs.langmuir.5b01124

9. Shevchuk A. I., Frolenkov G. I., Sánchez D., James P. S., Freedman N., Lab M. J. et al. Imaging proteins in membranes of living cells by high-resolution scanning ion conductance microscopy. Angew. Chem. Int. Ed. 2006;45(14):2212–2216. https://doi.org/10.1002/anie.200503915

10. Nashimoto Y., Takahashi Y., Ida H., Matsumae Y., Ino K., Shiku H. Nanoscale imaging of an unlabeled secretory protein in living cells using scanning ion conductance microscopy. Anal. Chem. 2015;87(5):2542–2545. https://doi.org/10.1021/ac5046388

11. Koya A. N., Zhu X., Ohannesian N., Yanik A. A., Alabastri A., Zaccaria R. P. Nanoporous metals: From plasmonic properties to applications in enhanced spectroscopy and photocatalysis. ACS Nano. 2021;15(4):6038–6060. https://doi.org/10.1021/acsnano.0c10945

12. Bandarenka H. V., Khinevich N. V., Burko A. A., Redko S. V., Zavatski S. A., Shapel U. A. et al. 3D silver dendrites for single-molecule imaging by surface-enhanced Raman spectroscopy. ChemNanoMat. 2021;7(2):141–149. https://doi.org/10.1002/cnma.202000521

13. Karooby E., Sahbafar H., Heris M. H., Hadi A., Eskandari V. Identification of low concentrations of flucytosine drug using a surface-enhanced Raman scattering (SERS)-active filter paper substrate. Plasmonics. 2024;19(2):855–863. https://doi.org/10.1007/s11468-023-02042-1

14. Dubkov S., Overchenko A., Novikov D., Kolmogorov V., Volkova L., Gorelkin P. et al. Single-cell analysis with silver-coated pipette by combined SERS and SICM. Cells. 2023;12(21):2521. https://doi.org/10.3390/cells12212521

15. Fletcher N. D., Lieb H. C., Mullaugh K. M. Stability of silver nanoparticle sulfidation products. Sci. Total Environ. 2019;648:854–860. https://doi.org/10.1016/j.scitotenv.2018.08.239

16. Zienkiewicz-Strzalka M., Blachnio M. Nitrogenous bases in relation to the colloidal silver phase: Adsorption kinetic, and morphology investigation. Appl. Sci. 2023;13(6):3696. https://doi.org/10.3390/app13063696

17. Roldán M. V., Pellegri N. S., Sanctis O. A. de. Optical response of silver nanoparticles stabilized by amines to LSPR based sensors. Procedia Mater. Sci. 2012;1:594–600. https://doi.org/10.1016/j.mspro.2012.06.080

18. Lugaresi O., Perales-Rondón J. V., Minguzzi A., Solla-Gullón J., Rondini S., Feliu J. M., Sánchez-Sánchez C. M. Rapid screening of silver nanoparticles for the catalytic degradation of chlorinated pollutants in water. Appl. Catal., B. 2015;163:554–563. https://doi.org/10.1016/j.apcatb.2014.08.030

19. Keast V. J. Corrosion processes of silver nanoparticles. Appl. Nanosci. 2022;12(6):1859–1868. https://doi.org/10.1007/s13204-022-02462-1

Information about the publication

UDK: 539.216.1:537.533.35
Publication type: Scientific article
Source lang: Russian
DOI: 10.24151/1561-5405-2025-30-6-741-753
RISC id: SACXMB

JATS XML